Computational analysis of crystallization trials
نویسندگان
چکیده
منابع مشابه
Computational analysis of crystallization trials.
A system for the automatic categorization of the results of crystallization experiments generated by robotic screening is presented. Images from robotically generated crystallization screens are taken at preset time intervals and analyzed by the computer program Crystal Experiment Evaluation Program (CEEP). This program attempts to automatically categorize the individual crystal experiments int...
متن کاملComputational crystallization.
Crystallization is a key step in macromolecular structure determination by crystallography. While a robust theoretical treatment of the process is available, due to the complexity of the system, the experimental process is still largely one of trial and error. In this article, efforts in the field are discussed together with a theoretical underpinning using a solubility phase diagram. Prior kno...
متن کاملAutomated Scoring of Crystallization Trials
Recently, the use of robotics and parallel techniques for protein production and crystallization is becoming commonplace among structural genomics initiatives, whose contributions amount to 73% of newly solved structures each year. Despite the strides made in increasing physical experimental throughput, the act of finding just a few crystals among potentially thousands of crystallization experi...
متن کاملTowards the automated evaluation of crystallization trials.
A method to evaluate images from crystallization experiments is described. Image discontinuities are used to determine boundaries of artifacts in the images and these are then considered as individual objects. This allows the edge of the drop to be identified and any objects outside this ignored. Each object is evaluated in terms of a number of attributes related to its size and shape, the curv...
متن کاملEstablishing a training set through the visual analysis of crystallization trials. Part I: ∼150 000 images
Structural crystallography aims to provide a three-dimensional representation of macromolecules. Many parts of the multistep process to produce the three-dimensional structural model have been automated, especially through various structural genomics projects. A key step is the production of crystals for diffraction. The target macromolecule is combined with a large and chemically diverse set o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Crystallographica Section D Biological Crystallography
سال: 2002
ISSN: 0907-4449
DOI: 10.1107/s0907444902016840